Joanne and her husband, Luke, are a EVO hunting vigilantes, attempting to avenge the death of their son at Odessa. In the course of the series, her desire for vengeance grows while Luke's wanes. Luke then evolves himself, and he becomes the very thing that they hunt, so she leaves him rather than kills him.
"Brave New World (Reborn)" June 13th, Odessa Texas, Joanne and Luke are at the Unity Summit with their son, Dennis, when the explosion occurred. Dennis could not be found, and the frantic parents searched for him. When his body is finally discovered, they are devastated. When she later discovers that Luke had found and rescued on EVO that day, but not Dennis, she attacked the EVO Luke saved. When he knocks her down and Luke attacks him, he begins to use his power on Luke and freeze him to death. Joanne then stabs him, and smiles as she watches him die.
One year later, Joanne is seen running down a street in Chicago Illinois, chasing an EVO. She pursues him into a secret meeting with other EVOs where Luke has infiltrated the meeting. They draw weapons and kill all the EVOs there and then burn the building down, "for Dennis." When Luke tells her one got away, they follow him to Moe's ice cream shop in Carbondale, waiting to see if Tommy will show up. When she goes to the restroom, by the time she comes back to the table, she sees Tommy with Luke. She shows him her weapon and takes him out back. When Emily appears and Joannes says they will have to take them both, Tommy uses his ability to teleport the Collins' away.
In "Odessa" Joanne and Luke are seen trapped inside the mysterious, windowless room that Tommy Teleported them to. She fires her gun, but the bullet ricochets off the walls. She gets ready to try it again but Luke stops her before she kills them both. They begin to argue, he blames them being stuck there on her because Luke wanted to go after El Vengador but Joanne decided against it. Joanne starts talking about how bad the EVO's are, she believes they are doing the right thing. Luke eventually figures out that if they turn off the lights they may see what they shouldn't, and shoots out the lights. He finds a one way observational window and they break through it to get out of the room. They find a man in the hall and force him to lead them to the exit, which unfortunately is through ha room full for technicians and security. Without thinking, Joanne begins shooting, even though Luke tries to stop her. When she doesn't listen, he too, returns fire to protect her from the security detail. They kill everyone in the room and once outside they steal Noah Bennet's car, where Luke prevents her from killing Quentin.
In "Under the Mask" Joanne is somewhere in Texas with Luke in Noah Bennet's car, going through all of the EVO profiles that Bennet had, she calls it a "hit list". Luke seems unhappy with the find, but she is delighted. When the car then shorts out they are forced to walk. They stop at a diner to eat, and Luke begins wondering why they're still hunting, they could go home. Joanne tells him it's the right thing to do, then she orders him a drink but he declines. When she offers him a choice from the hit llist, Luke wants to go to LA and Joanne knows it's because he and Dennis loved the ocean. She doesn't see as Luke's knife begins heating up. He complains of a headache and she drives them to the Lido motel. Joanne goes to get the room while Luke stays outside for some fresh air, it is then, when she is away that Luke's power first manifests.
In "The Needs of the Many", Joanne knocks on the door of a EVO target, he opens the door and she shoots him in the head, then compliments herself on the "pretty shot." Joanne and Luke goes into his house, and is more reluctant than ever. Luke tells her that he had a family but Joanne doesn't care, she calls him, and all EVOs, monsters.
At the next motel, Joanne enters the room and Luke tells her that he wants to stop hunting. Joanne doesn't, she feels his head and it's extremely hot, and she says he'll feel better about it when he feels better, and she gets Luke some water. She goes out hunting alone.
When they later leave, she begins reminiscing about the first time they killed a EVO. Luke tells her that it never made him feel better, but he saw how soundly she slept after killing that first EVO even though it made him sick. He told her they were lost and then using his new power, sets the list of EVO names on fire. Joanne slams the car to a stop and points her gun at him but can't quite pull the trigger, she says, "I really did love you," and then gets out the car and walks away.
In "June 13th - Part One", in a flashback, Joanne and Luke are in at a hotel in Odessa, with their son, Dennis, he has an illness, Solar Urticaria, an allergy to sunlight which causes a severe rash or even anaphylactic shock when he is exposed to the sun. Joanne appears as a doting, nurturing, and overprotective mother. They want to go to the Summit, hoping that one of the EVOs can help Dennis live a normal life. They all arrive at the Odessa Unity Summit, she is worried it's a bad idea, but Luke assures her that it isn't. The sky goes dark and Joanne loses sight of Dennis, the building explodes, and she panics.
In "June 13th - Part Two", Joanne and Luke put a picture of Dennis on a board at the summit for the people that are still missing. She and Luke must wait to see if Dennis is found, they are no longer allowed to search for themselves. Eventually, a worker brings in his body, Dennis is dead, they utterly devastated, and begin to cry.
Later Joanne and Luke return to their hotel room when the Ice Man knocks on their door, he wants to thank Luke for saving him, Joanne becomes irrational when she discovers her husband saved "one of them" instead of Dennis. Luke tries to explain that he had to help, but Joanne attacks the man who pushes her off, knocking her down. Luke then snaps and attacks the man who grabs Luke by his throat and begins to freeze him, but Joanne stabs him repeatedly with scissors, killing him - and then smiles.
In "Sundae, Bloody Sundae", Joanne enters Moe's and sits down, Emily approaches her, and then Jo points a gun at the girl. Caspar is suspicious of her, so he drops several pennies on the floor but right before she picks it up, she can see Emily warning Caspar off, so she pulls the gun out on both of them and closes the shop up. She calls Tommy from Emily's phone, and tells him that they're at Moe's. Caspar tries to talk her into putting the gun down, but then Tommy arrives. Caspar throws his suitcase containing all of his pennies into the air and they go everywhere, Joanne shoots him in the head. Luke enters the shop just as she is about to shoot Emily, and tries to talk her down. They argue, and she aims at Emily, but the next thing she knows, Tommy and Emily are gone. Joanne seems confused and she runs out the shop with Luke in pursuit. She runs through an ally and M.F. Harris pulls up and tells her to get in.
In "Send in the Clones", Joanne is taken to Erica Kravid. Erica recognizes her obsession and her skills and all that they could offer each other. Joanne is offered a job and a alliance with Renautas. She gives Joanne her first assignment, her next target is Malina and Luke Collins.
In "Company Woman", Joanne is sent to Union Wells High School to kill Malina. Shortly after she arrives, she notices Luke, Quentin, and Malina. Without hesitation, she fires into the crowd, she misses Malina and everyone in the gym panics, and runs toward the exit, causing a lot of confusion. Someone bumps into Joanne knocking the gun out of her hand. Luke calls to her, begging her to stop, but she won't, she says she can't.
By the time she picks her gun back up, the sun has risen and Luke's Power is working, he attempts to calm Joanne down but it doesn't work, she fires twice at Malina. Immediately and instinctively, Luke blasts her with his power. Nothing remains of Joanne but ash.
- "I really did love you." (The Needs of the Many)
- Joanna is the worst antagonist, due to her bad motivation to wanting to kill people.
- Joanne has separated from Luke, due to discovering his nature as an EVO.
- Joanne's first kill was the Ice Man.
- Joanne was killed by her husband.